IVF TREATMENT- In Vitro Fertilisation
What is IVF TREATMENT?
Sperm collection and processing should be done very carefully to avoid infection. Before this IVF treatment can be designed, the husband must have a test product of the sperm to see the integrity and biology of the sperm, and it is time to survive. Now the husband should take the egg and give the sperm sample within 2 hours. If he thinks this may be a problem in these tense times, he should have done it in the previous days, which may have saved Cryo. This also applies to husbands working abroad or undergoing surgery / radio, chemotherapy. If the husband is unable to model through masturbation, he can be given a non-toxic condom.
The specifically prepared sperms and the incubated eggs are combined in a test tube or petri dish in prescribed number ratio and left for few hours for them to unite.
IVF TREATMENT
In Vitro Fertilisation
The culture dishes are kept in a modular chamber which holds the gaseous environment very strictly and these are kept in turn in a CO2 incubator. Our IVF lab utilizes TRIGAS (O2, CO2, N2 mixture) incubator instead of CO2 alone, since the presence of 5 % O2 improves the culture Conditions and integrity of embryos.
After 18 hours, they are examined under stereo zoom microscope for fertilization which is obvious if two pronucleus are seen clearly . Each pronucleus denote the decondensation of male and female nucleus (DNA). Once they are identified fertilization is confirmed. This day could be accounted as Day 1.