ICSI TREATMENT – Intracytoplasmic Sperm Injection
This is a procedure in which a sperm is injected in to the cytoplasm of one egg using micro injection needle with the help of micromanipulators under inverted microscopes with X 400 magnification. The main advantage of the Method is that, it needs only very few sperms equivalent with the number of eggs obtained. This stands as the first line of treatment in extreme male infertility like severe Oligospermia, asthenospermia etc. Surgically extracted few sperms are ideally used for this procedure in males who are otherwise Azoospermic (nil sperms in the ejaculate).
Sperms are immobilized using PVP (latest being LASER immobilization) and they are aspirated singly in the microinjection needle and the egg is held using a holding pipette. Then the loaded sperm is injected into the cytoplasm of the egg. All these techniques need great deal of skill and hitech equipments. By doing this several barriers of fertilization are overcome and fertilization occur in almost all eggs injected. The overall success rates is around 41% in day 3 transfer and 63% success in day 5/6 transfers in our center.
Before undertaking this treatment males are supposed to subject themselves for blood karyotyping test which must be normal as a pre requisite for ICSI.
SURGICAL SPERM EXTRACTION
This is done when there is no sperms in the ejaculate. Very few live sperms or spermatids (younger sperms) could also help in doing ICSI and getting babies. Methods explained as below are done according to the need of the male partner.